منابع مشابه
Crystallization of Goat p - Lactoglobulin
as 'goat lactoglobulin' gave a single peak on electrophoresis at pH 8.0 in barbiturate buffer, but three boundaries separated at pH 7-0 and at least two at pH 4-2-6-0 after 2 hr. at 4-5 v/cm., I= 01. The two main components had their isoelectric points at pH 5-2 and 5-9 respectively in sodium acetate buffer, I= 0-1. 'Goat lactoglobulin' thus behaved in much the same way as crystalline bovine ,B...
متن کاملStable silencing of β-lactoglobulin (BLG) gene by lentivirus-mediated RNAi in goat fetal fibroblasts
β-lactoglobulin (BLG), a dominant allergen in goat milk, is difficult to remove by traditional biochemical methods. Its elimination from goat milk by genetic modification therefore poses a major challenge for modern goat breeders. A shRNA targeting BLG mRNA with high interference efficiency was identified, with which lentiviral vectors were used for mediating stable shRNA interference in goat-f...
متن کاملRapid communication: Polymorphism in the goat β-lactoglobulin proximal promoter region1
Polymorphism. Polymorphism was detected within a 710-bp-long PCR amplification product of the goat βlactoglobulin (BLG) gene, comprising 588 bp of the proximal promoter region and 122 bp of exon 1, using the base excision sequence scanning method (BESS TScan; Epicentre Technologies, Madison, WI). A total of forty genomic DNA samples from animals of different Spanish (Canaria, Malagueña, Payoya,...
متن کاملStructure of bovine β-lactoglobulin-lactoferrin coacervates.
Lactoferrin (LF) and β-lactoglobulin (BLG) are among the protein pairs that exhibit heteroprotein coacervation, a unique and relatively unexamined type of liquid-liquid phase separation (LLPS). In prior work we found that LF and BLG undergo coacervation at highly constrained conditions of pH, ionic strength and protein stoichiometry. The molar stoichiometry in coacervate and supernatant is LF :...
متن کاملInteractions of β-lactoglobulin with Cationic Surfactants: Spectroscopy Study
The interactions of β-lactoglobulin AB in the presence of cationic surfactants such as Cetyltrimethylammonium bromide and Cetyltrimethylammonium p-Toluenesulfonate have been investigated using a variety of experimental techniques such as conductivity, UV-Vis spectrophotometry and fluorimetry. The conductivity of surfactants aqueous solutions with β-lactoglobulin shows that the cmc of cationic s...
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ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1954
ISSN: 0306-3283
DOI: 10.1042/bj0580332